Gray et al (2013) Activation of conventional protein kinase C (PKC), and in particular PKC ? is critically involved in the generation of human neutrophil extracellular traps. J Inflamm (Lond). 21;10(1):12

Activation of conventional protein kinase C (PKC), and in particular PKC ? is critically involved in the generation of human neutrophil extracellular traps.

Gray, R. D, Mackellar, A., Li, F., Hiersemenzel, K., Haslett, C., Davidson, D. J.* Rossi, A. G*.
J Inflamm (Lond) (2013) 21;10(1):12.    PubMed     Full Free Text

* denotes equal contribution

Davidson lab supported by funding from: Wellcome Trust and MRC

BACKGROUND: Activation of NADPH oxidase is required for neutrophil extracellular trap (NET) formation. Protein kinase C (PKC) is an upstream mediator of NADPH oxidase activation and thus likely to have a role in NET formation.
METHODS: Pharmacological inhibitors were used to block PKC activity in neutrophils harvested from healthy donor blood.
RESULTS: Pan PKC inhibition with Ro-31-8220 (p<0.001), conventional PKC inhibition with Go 6976 (p<0.001) and specific PKCbeta inhibition with LY333531 (p<0.01) blocked NET formation in response to PMA. Inhibition of novel and atypical PKC had no effect. LY333531 blocked NET induction by the diacylglycerol analogue OAG (conventional PKC activator) (p<0.001). CONCLUSIONS: Conventional PKCs have a prominent role in NET formation. Furthermore PKCbeta is the major isoform implicated in NET formation. PMID: 23514610

 

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